Journal: Frontiers in Endocrinology

Article Title: Serum fibroblast growth factor 1 and its association with pancreatic beta cell function and insulin sensitivity in adults with glucose intolerance

doi: 10.3389/fendo.2023.1198311

Figure Lengend Snippet: Clinical characteristics of individuals with undetectable and detectable serum FGF1 levels.

Article Snippet: Serum FGF1 levels were examined using an enzyme-linked immunosorbent assay (catalog no. DFA00B; R&D Systems, Inc., Minneapolis, MN USA).

Techniques:

Journal: Frontiers in Endocrinology

Article Title: Serum fibroblast growth factor 1 and its association with pancreatic beta cell function and insulin sensitivity in adults with glucose intolerance

doi: 10.3389/fendo.2023.1198311

Figure Lengend Snippet: Metabolic parameters of individuals with undetectable (group 1), low (group 2), and high (group 3) serum FGF1 levels.

Article Snippet: Serum FGF1 levels were examined using an enzyme-linked immunosorbent assay (catalog no. DFA00B; R&D Systems, Inc., Minneapolis, MN USA).

Techniques:

Journal: Frontiers in Endocrinology

Article Title: Serum fibroblast growth factor 1 and its association with pancreatic beta cell function and insulin sensitivity in adults with glucose intolerance

doi: 10.3389/fendo.2023.1198311

Figure Lengend Snippet: Metabolic parameters of individuals with undetectable (group 1), low (group 2), and high (group 3) serum FGF1 levels. DI, disposition index; IGI, insulinogenic index; ISI, Matsuda insulin sensitivity index.

Article Snippet: Serum FGF1 levels were examined using an enzyme-linked immunosorbent assay (catalog no. DFA00B; R&D Systems, Inc., Minneapolis, MN USA).

Techniques:

Journal: Frontiers in Endocrinology

Article Title: Serum fibroblast growth factor 1 and its association with pancreatic beta cell function and insulin sensitivity in adults with glucose intolerance

doi: 10.3389/fendo.2023.1198311

Figure Lengend Snippet: Univariable and multivariable analysis of the association between serum FGF1 levels and metabolic parameters using a Tobit regression model.

Article Snippet: Serum FGF1 levels were examined using an enzyme-linked immunosorbent assay (catalog no. DFA00B; R&D Systems, Inc., Minneapolis, MN USA).

Techniques:

Journal: Frontiers in Endocrinology

Article Title: Serum fibroblast growth factor 1 and its association with pancreatic beta cell function and insulin sensitivity in adults with glucose intolerance

doi: 10.3389/fendo.2023.1198311

Figure Lengend Snippet: Box plot of the estimated log-transformed serum FGF1 level according to the quartile of each parameter using a Tobit regression model adjusted by age and sex. DI, disposition index; IGI, insulinogenic index; ISI, Matsuda insulin sensitivity index.

Article Snippet: Serum FGF1 levels were examined using an enzyme-linked immunosorbent assay (catalog no. DFA00B; R&D Systems, Inc., Minneapolis, MN USA).

Techniques: Transformation Assay

Journal: iScience

Article Title: Sebocytes contribute to melasma onset

doi: 10.1016/j.isci.2022.103871

Figure Lengend Snippet:

Article Snippet: Human FGF1/FGF basic ELISA Kit , Origene Technologies Inc. , Cat#EA102179.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Ex Vivo, Software, Modification

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Loss of NgBR causes neuronal damage through decreasing KAT7-mediated RFX1 acetylation and FGF1 expression

doi: 10.1007/s00018-025-05660-6

Figure Lengend Snippet: NgBR deficiency leads to the downregulation of FGF1 expression and secretion. A Expression clustering heatmap analysis of differentially expressed genes related to the PI3K-AKT pathway shown in Fig. B. B‒D SH-SY5Y cells were transfected with the indicated siRNAs for 48 h. Then, the samples were subjected to qRT‒PCR ( B ) or immunoblotting ( C ). The relative protein level of FGF1 to that of β-actin in ( C ) was analyzed as shown in D. n = 3, * P < 0.05, ** P < 0.01, *** P < 0.001. E SH-SY5Y cells were transfected with Flag or Flag-NgBR for 48 h, after which the cell lysates were subjected to immunoblot analysis. F The protein levels of FGF1 relative to those of β-actin in ( E ) were analyzed. n = 3, ** P < 0.01. G N2a cells were transfected with the indicated siRNAs for 48 h. Then, the cell lysates were subjected to immunoblot analysis. H The protein level of FGF1 relative to that of β-actin in ( G ) was analyzed. n = 3, * P < 0.05, ** P < 0.01. I MES23.5 cells were transfected with the indicated siRNAs for 48 h. Then, the cell lysates were subjected to immunoblot analysis. J The protein level of FGF1 relative to that of β-actin in ( I ) was analyzed. n = 3, * P < 0.05. K Nus1 flox/flox primary cortical neurons were infected with LV-Ctrl or LV-Cre and cultured for 6 days. Then, the cell lysates were subjected to immunoblot analysis. L The protein level of FGF1 relative to that of β-actin in ( K ) was analyzed. n = 3, ** P < 0.01. M-N SH-SY5Y cells were transfected with the indicated siRNAs for 24 h. Then, the cells were cultured with serum-free medium for 24 h, and the FGF1 content in the culture medium ( M ) and cell lysate ( N ) was detected via an ELISA kit. n = 3, * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: SH-SY5Y cells were inoculated into 24-well plates and treated with siRNAs against NUS1 for 24 h. Then, the cells were cultured with serum-free medium for 24 h, and 100 μL of the FGF1 content in the culture medium or in cell lysate was measured with an ELISA kit (EK0339, BOSTER, Wuhan, China).

Techniques: Expressing, Transfection, Western Blot, Infection, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: Kidney international

Article Title: Fibroblast growth factor 1 ameliorates diabetic nephropathy by an anti-inflammatory mechanism

doi: 10.1016/j.kint.2017.05.013

Figure Lengend Snippet: (a,b) The expression of proinflammatory cytokines (TNF-α and IL-6) determined by enzyme-linked immunosorbent assay in murine podocyte cells (MPCs) that have been pretreated with FGF1 (1, 10, or 100 ng/ml) for 2 hours, followed by treatment with HG (25 mM) for an additional 24 hours; values reported as mean ± SEM, ##P < 0.01 versus LG, *P < 0.05, **P < 0.01, and ***P < 0.001 versus HG (N = 3). (c) Effects of FGF1 pretreatment (1, 10, or 100 ng/ml for 1 hour) of MPCs on HG-induced (2 hours) activation of IKKα/β and JNK. (d) Immunofluorescent localization of NF-κB p65 subunit (red) in MPCs: Ctrl, mannitol (osmotic control), HG, HG+FGF1 (100 ng/ml); nuclei stained with fluorescent DAPI (blue). Ctrl, control; DAPI, 4′,6-diamidino-2-phenylindole; FGF1, fibroblast growth factor 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HG, high glucose; p-IKKα/β; JNK, c-Jun N-terminal kinase; IL-6, interleukin-6; LG, low glucose; NF-κB, nuclear factor-κB; p-IKKα/β, phosphorylation of inhibitor of nuclear factor kappa-B kinase subunit alpha/beta; p-JNK, phosphorylation of c-Jun N-terminal kinase; TNF-α, tumor necrosis factor-α. To optimize viewing of this image, please see the online version of this article at www.kidney-international.org.

Article Snippet: The medium was collected and diluted 5-fold with 0.01 mol/l PBS (pH = 7.0–7.2) and assayed using mouse FGF1 enzyme-linked immunosorbent assay kit (Cloud-Clone Corp.), in accordance to the manufacturer’s protocol, and absorbance measured at 450 nm.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Activation Assay, Staining